Poage, Miranda and Le Martret, Benedicte and Jansen, Marcel A.K. and Nugent, Gregory D. and Dix, Philip
(2011)
Modification of reactive oxygen species scavenging capacity
of chloroplasts through plastid transformation.
Plant Molecular Biology, 76.
pp. 371-384.
Abstract
Reactive oxygen species (ROS), including
superoxide anions, hydrogen peroxide and hydroxyl radicals
are generated through normal biochemical processes,
but their production is increased by abiotic stresses. The
prospects for enhancing ROS scavenging, and hence stress
tolerance, by direct gene expression in a vulnerable cell
compartment, the chloroplast, have been explored in
tobacco. Several plastid transformants were generated
which contained either a Nicotiana mitochondrial superoxide
dismutase (MnSOD) or an Escherichia coli glutathione
reductase (gor) gene. MnSOD lines had a three-fold
increase in MnSOD activity, but interestingly a five to
nine-fold increase in total chloroplast SOD activity. Gor
transgenic lines had up to 6 times higher GR activity and
up to 8 times total glutathione levels compared to wild type
tobacco. Photosynthetic capacity of transplastomic plants,
as measured by chlorophyll content and variable fluorescence
of PSII was equivalent to non-transformed plants.
The response of these transplastomic lines to several
applied stresses was examined. In a number of cases
improved stress tolerance was observed. Examples include
enhanced methyl viologen (Paraquat)-induced oxidative
stress tolerance in Mn-superoxidase dismutase overexpressing
plants, improved heavy metal tolerance in glutathione
reductase expressing lines, and improved tolerance
to UV-B radiation in both sets of plants.
| Item Type: |
Article
|
| Keywords: |
Chloroplast transformation; Superoxide
dismutase; Glutathione reductase; Abiotic stress; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
9722 |
| Identification Number: |
https://doi.org/10.1007/s11103-011-9784-y |
| Depositing User: |
Prof. Philip J. Dix
|
| Date Deposited: |
26 Jul 2018 16:00 |
| Journal or Publication Title: |
Plant Molecular Biology |
| Publisher: |
Springer |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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