Deane, C.R. and Fuller, M.P. and Dix, Philip
(1995)
Selection of hydroxyproline-resistant proline-accumulating mutants of cauliflower.
C.R. Deane, M .P. Fuller' & P.J. Dix.
Euphytica, 85.
pp. 329-334.
Abstract
A procedure is described by which hydroxyproline-resistant lines could be selected from regenerating curd tissue
of cauliflower. Mutagenesis was by N-nitroso-N-ethylurea, supplied as a drop of 0 .3 mM solution on each 3 mm
diameter curd piece. The mutagen generated numerous morphological and pigment mutations without significantly
affecting shoot regeneration from explants . Thirty one resistant shoots were recovered from more than six thousand
explants mutagenised on regeneration medium supplemented with 3 mM hydroxyproline, while none was obtained
from a similar number of non-mutagenised controls . Out of twenty-three resistant shoots which survived subculture,
only one showed consistently elevated levels of endogenous proline . During early shoot culture passages, proline
levels were 3 .6-4.7 times higher than controls, but this was reduced to 1 .6 times after 10-12 culture passages in
the absence of hydroxyproline . Possible reasons for this decline are discussed . Leaf strip assays suggest resistant
shoots may be chimeras and current efforts are directed towards regenerating solid mutants from resistant sectors .
These will then be evaluated for any alteration in frost tolerance .
| Item Type: |
Article
|
| Keywords: |
curd culture; frost tolerance; N-nitroso-N-ethylurea; proline accumulation; mutagenesis; |
| Academic Unit: |
Faculty of Science and Engineering > Biology |
| Item ID: |
9728 |
| Depositing User: |
Prof. Philip J. Dix
|
| Date Deposited: |
27 Jul 2018 13:02 |
| Journal or Publication Title: |
Euphytica |
| Publisher: |
luwer Academic Publishers |
| Refereed: |
Yes |
| URI: |
|
| Use Licence: |
This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available
here |
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