Timmons, Aileen Mary and Dix, Philip (1993) Ultrastructure of Lycopersicon peruvianum leaf explants and streptomycin-resistant shoots on medium containing streptomycin. Plant Cell, Tissue and Organ Culture, 34. pp. 115-123.

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Abstract

The effect of streptomycin on morphogenic explants of Lycopersicon peruvianum Mill. was examined microscopically at both the light and ultrastructural level. Early stages in shoot regeneration from leaf explants were distinguished as meristematic tissue at both levels. Small starch grains were observed in the plastids in this tissue but not in plastids in regenerated shoots. In the presence of streptomycin, adventitious shoot regeneration from sensitive leaf strips was inhibited. Large layered bodies were observed within the plastids of sensitive leaf tissue, suggesting the disruption of thylakoid membrane formation. Streptomycin resistant L. peruvianum lines, as well as a chlorophyll-deficient line, were also examined microscopically. The chloroplasts of newly regenerated streptomycin resistant shoots contained well developed internal membranes and conspicuous starch grains. Cells containing a mixture ofresistant and sensitive plastids were not observed. The plastids in chlorophyll-deficient tissue completely lacked thylakoid membranes, although small vesicles and intraplastid bodies were seen within the stroma.

Item Type:	Article
Keywords:	adventitious shoots; antibiotic resistance; chloroplast mutations; plastid segregation;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	9731
Depositing User:	Prof. Philip J. Dix
Date Deposited:	31 Jul 2018 11:31
Journal or Publication Title:	Plant Cell, Tissue and Organ Culture
Publisher:	Kluwer Academic Publishers
Refereed:	Yes
URI:
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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