Share and Export
Share and Export
Kavanagh, Kevin, Walsh, M. and Whittaker, Peter A. (1991) Enhanced intraspecific protoplast fusion in yeast. FEMS Microbiology Letters, 81 (3). pp. 283-286. ISSN 0378-1097
Preview
KK-Enhanced-1991.pdf
Download (330kB) | Preview
Abstract
Intraspecific hybrid production from the polyethylene glycol induced fusion of yeast protoplasts was greatly increased when calcium propionate was included as the source of the requisite Ca2+. The use of calcium propionate, as opposed to the more commonly employed calcium chloride, resulted in substantially greater yields of hybrids from intraspecific fusions of protoplasts of Saccharomyces cerevisiae and Candida albicans. It is postulated that the ability of calcium propionate to enhance the fusion frequency is due to the anion binding to the etheric oxygen of PEG and potentiating the fusogenicity of the polymer.
| Item Type: | Article |
|---|---|
| Keywords: | Protoplast fusion; Yeast; Candida albicans; Saccharomyces cerevisiae; |
| Academic Unit: | Faculty of Science and Engineering > Biology |
| Item ID: | 11127 |
| Identification Number: | 10.1111/j.1574-6968.1991.tb04773.x |
| Depositing User: | Dr. Kevin Kavanagh |
| Date Deposited: | 04 Oct 2019 13:28 |
| Journal or Publication Title: | FEMS Microbiology Letters |
| Publisher: | Elsevier B.V. |
| Refereed: | Yes |
| Related URLs: | |
| URI: | https://mural.maynoothuniversity.ie/id/eprint/11127 |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
Repository Staff Only (login required)
- Item control page
Downloads
Downloads per month over past year
