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Dix, Philip, Joo, Ferenc and Maliga, Pal (1977) A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin. Molecular and General Genetics MGG, 157. pp. 285-290. ISSN 0026-8925
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Abstract
Cell lines resistant to 50 gg ml- 1 kanamycin sulphate were isolated from cell suspension cultures initiated from a haploid Nicotiana sylvestris
plant. One line, KR103, has been studied in detail.
Resistance of this line was shown to be stable in
the absence of the drug. KR103 was found also to
be resistant to streptomycin, another inhibitor of 70S
ribosomal protein synthesis.
Both KR103 and the sensitive line convert kanamycin, but not streptomycin, to a form which is no
longer effective in a bacterial bioassay, while maintaining its toxicity for sensitive plant cells.
KR103 is defective in morphogenesis and plastid
development.
| Item Type: | Article |
|---|---|
| Keywords: | cell line; Nicotiana sylvestris; resistance; kanamycin; streptomycin; |
| Academic Unit: | Faculty of Science and Engineering > Biology |
| Item ID: | 13908 |
| Depositing User: | Prof. Philip J. Dix |
| Date Deposited: | 01 Feb 2021 14:10 |
| Journal or Publication Title: | Molecular and General Genetics MGG |
| Publisher: | Springer Verlag |
| Refereed: | Yes |
| Related URLs: | |
| URI: | https://mural.maynoothuniversity.ie/id/eprint/13908 |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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