The human DEAD box protein DDX3X has known roles in mRNA translation regulation and anti-viral innate immune signalling. It is targeted by a diverse range of viruses, and there is the potential for inhibitors of DDX3X enzymatic activity to be utilised as broadspectrum antiviral drugs. However, it is unknown how viral infection alters the interactions between DDX3X and the host transcriptome, and whether the host translation regulation function of DDX3X has any role in anti-viral immunity. Using high-throughput techniques, we characterise features of and changes in host mRNA binding by DDX3X in response to Sendai Virus infection. We determine that DDX3X both binds and regulates translation of the mRNA of IFNB1, a key anti-viral innate immune signalling gene that is also regulated transcriptionally by DDX3X in the latter's immune signalling role. Additionally, we identify and confirm several targets of DDX3X regulation, with mRNA secondary structure emerging as a key factor in identifying and characterising putative targets. Finally, we determine that DDX3X binds SeV mRNA and regulates translation of some SeV genes, which contributes to the landscape of known DDX3X-virus interactions.