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Timmons, A.M. and Dix, Philip (1991) Influence of ploidy on plastome mutagenesis in Nicotiana. A.M. Timmons* and P.J. Dix. Molecular and General Genetics, 227 (2). pp. 330-333.
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Abstract
A clear influence of ploidy was observed on
the frequency of both spontaneous and nitroso-methylurea
(NMU) induced, streptomycin-resistant, adventitious
shoots developing on leaf explants of Nicotiana
tabacum and N. plumbaginifolia. At nearly all NMU levels
employed a significantly higher yield of resistant
shoots was obtained from haploid compared with diploid
leaf strips. At 1 mM NMU the differences were
not significant and were absent when a high (1000 mg/1)
selective concentration of streptomycin sulphate was
used. The influence of ploidy is discussed in relation
to the possible effect of plastome copy number on mutagenesis
and sorting out of resistant plastids.
| Item Type: | Article |
|---|---|
| Keywords: | Mutagenesis; Nitroso-methylurea; Plastome; Ploidy; Nicotiana; |
| Academic Unit: | Faculty of Science and Engineering > Biology |
| Item ID: | 9986 |
| Depositing User: | Prof. Philip J. Dix |
| Date Deposited: | 20 Sep 2018 13:05 |
| Journal or Publication Title: | Molecular and General Genetics |
| Publisher: | Springer International |
| Refereed: | Yes |
| URI: | https://mural.maynoothuniversity.ie/id/eprint/9986 |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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