Nguyen, T.T. and Dix, Philip and Nugent, Gregory D. (2010) Transformation of potato via Agrobacterium coated microparticle bombardment. Biologia Plantarum, 54 (1). pp. 141-144. ISSN 006-3134

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Abstract

The transformation of potato (Solanum tuberosum L. cv. Désirée) was extended by the Agrobacterium-mediated biolistic method. Using this approach transgenic shoots could be obtained at a similar frequency to that achieved through conventional biolistics. Leaves from shoot cultures were bombarded with gold particles coated in Agrobacterium tumefaciens cells harboring a binary plasmid encoding three genes of interest in the T-DNA. Nine shoots were obtained from 20 shots, with selection of transgenic shoots on a series of media containing progressively increasing concentrations of hygromycin from 5 to 20 mg dm-3.

Item Type:	Article
Keywords:	potato; Agrobacterium coated microparticle bombardment; Agrobacterium transformation; ipt gene; Solanum tuberosum; transgenic potato;
Academic Unit:	Faculty of Science and Engineering > Biology
Item ID:	7371
Identification Number:	https://doi.org/10.1007/s10535-010-0022-2
Depositing User:	Prof. Philip J. Dix
Date Deposited:	24 Aug 2016 15:39
Journal or Publication Title:	Biologia Plantarum
Publisher:	Springer Verlag
Refereed:	No
URI:	http://link.springer.com
Use Licence:	This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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