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    Influence of ploidy on plastome mutagenesis in Nicotiana. A.M. Timmons* and P.J. Dix

    Timmons, A.M. and Dix, Philip (1991) Influence of ploidy on plastome mutagenesis in Nicotiana. A.M. Timmons* and P.J. Dix. Molecular and General Genetics, 227 (2). pp. 330-333.

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    A clear influence of ploidy was observed on the frequency of both spontaneous and nitroso-methylurea (NMU) induced, streptomycin-resistant, adventitious shoots developing on leaf explants of Nicotiana tabacum and N. plumbaginifolia. At nearly all NMU levels employed a significantly higher yield of resistant shoots was obtained from haploid compared with diploid leaf strips. At 1 mM NMU the differences were not significant and were absent when a high (1000 mg/1) selective concentration of streptomycin sulphate was used. The influence of ploidy is discussed in relation to the possible effect of plastome copy number on mutagenesis and sorting out of resistant plastids.

    Item Type: Article
    Keywords: Mutagenesis; Nitroso-methylurea; Plastome; Ploidy; Nicotiana;
    Academic Unit: Faculty of Science and Engineering > Biology
    Item ID: 9986
    Depositing User: Prof. Philip J. Dix
    Date Deposited: 20 Sep 2018 13:05
    Journal or Publication Title: Molecular and General Genetics
    Publisher: Springer International
    Refereed: Yes
      Use Licence: This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here

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