Furuta, Glenn T., Kagalwalla, Amir F., Lee, James J., Alumkal, Preeth, Maybruck, Brian T., Fillon, Sophie A., Masterson, Joanne C., Ochkur, Sergei I., Protheroe, Cheryl, Moore, Wendy, Pan, Zhaoxing, Amsden, Katie, Robinson, Zachary, Capocelli, Kelley E., Mukkada, Vince, Atkins, Dan, Fleischer, David, Hosford, Lindsay, Kwatia, Mark A., Schroeder, Shauna, Kelly, Caleb, Lovell, Mark, Melin-Aldana, Hector and Ackerman, Steven J. (2013) The oesophageal string test: a novel, minimally invasive method measures mucosal inflammation in eosinophilic oesophagitis. Gut, 62 (10). pp. 1395-1405. ISSN 1468-3288
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Abstract
Objective Eosinophil predominant inflammation
characterises histological features of eosinophilic
oesophagitis (EoE). Endoscopy with biopsy is currently
the only method to assess oesophageal mucosal
inflammation in EoE. We hypothesised that
measurements of luminal eosinophil-derived proteins
would correlate with oesophageal mucosal inflammation
in children with EoE.
Design The Enterotest diagnostic device was used to
develop an oesophageal string test (EST) as a minimally
invasive clinical device. EST samples and oesophageal
mucosal biopsies were obtained from children
undergoing upper endoscopy for clinically defined
indications. Eosinophil-derived proteins including
eosinophil secondary granule proteins (major basic
protein-1, eosinophil-derived neurotoxin, eosinophil
cationic protein, eosinophil peroxidase) and Charcot–
Leyden crystal protein/galectin-10 were measured by
ELISA in luminal effluents eluted from ESTs and extracts
of mucosal biopsies.
Results ESTs were performed in 41 children with active
EoE (n=14), EoE in remission (n=8), gastro-oesophageal
reflux disease (n=4) and controls with normal
oesophagus (n=15). EST measurement of eosinophilderived protein biomarkers significantly distinguished
between children with active EoE, treated EoE in
remission, gastro-oesophageal reflux disease and normal
oesophagus. Levels of luminal eosinophil-derived
proteins in EST samples significantly correlated with peak
and mean oesophageal eosinophils/high power field
(HPF), eosinophil peroxidase indices and levels of the
same eosinophil-derived proteins in extracts of
oesophageal biopsies.
Conclusions The presence of eosinophil-derived
proteins in luminal secretions is reflective of mucosal
inflammation in children with EoE. The EST is a novel,
minimally invasive device for measuring oesophageal
eosinophilic inflammation in children with EoE.
Item Type: | Article |
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Keywords: | oesophageal string test; novel; minimally invasive; mucosal inflammation; eosinophilic oesophagitis; |
Academic Unit: | Faculty of Science and Engineering > Biology |
Item ID: | 12488 |
Identification Number: | 10.1136/gutjnl-2012-303171 |
Depositing User: | Joanne Masterson |
Date Deposited: | 27 Feb 2020 14:20 |
Journal or Publication Title: | Gut |
Publisher: | BMJ Publishing Group |
Refereed: | Yes |
Related URLs: | |
URI: | https://mural.maynoothuniversity.ie/id/eprint/12488 |
Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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