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Reiber, Kathrin, Reeves, Emer P., Neville, Claire M., Winkler, Robert, Gebhardt, Peter, Kavanagh, Kevin and Doyle, Sean (2005) The expression of selected non-ribosomal peptide synthetases in Aspergillus fumigatus is controlled by the availability of free iron. FEMS Microbiology Letters, 248 (1). pp. 83-91. ISSN 0378-1097
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Abstract
Three non-ribosomal peptide synthetase genes, termed sidD, sidC and sidE, have been identified in Aspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of both sidD and C was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis. SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD (200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy.
| Item Type: | Article |
|---|---|
| Keywords: | NRPS; Iron; Siderophore; MALDI ToF; Secondary metabolites; Proteomics; 40-PPTase; |
| Academic Unit: | Faculty of Science and Engineering > Biology |
| Item ID: | 231 |
| Identification Number: | 10.1016/j.femsle.2005.05.028 |
| Depositing User: | Dr. Sean Doyle |
| Date Deposited: | 14 Jul 2005 |
| Journal or Publication Title: | FEMS Microbiology Letters |
| Publisher: | Elsevier B.V. |
| Refereed: | Yes |
| Funders: | Higher Education Authority (HEA) |
| Related URLs: | |
| URI: | https://mural.maynoothuniversity.ie/id/eprint/231 |
| Use Licence: | This item is available under a Creative Commons Attribution Non Commercial Share Alike Licence (CC BY-NC-SA). Details of this licence are available here |
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